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Electrospray Ionization (ESI) Mass Spectrometry01:12

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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A stepwise surface ionization method for ion mobility spectrometry.

Jianhua Lin1,2, Xiaoguang Gao1, Jian Jia1

  • 1State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, China.

Rapid Communications in Mass Spectrometry : RCM
|July 15, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel surface ionization-ion mobility spectrometry method for detecting trimethylamine in breath. The technique offers high sensitivity and humidity resistance, promising for early disease detection.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Respiratory Medicine

Background:

  • Early disease detection, such as kidney disease, can be achieved by monitoring organic nitrogen compounds in exhaled breath.
  • Detecting trace disease markers in complex, humid exhaled breath presents significant analytical challenges.
  • Surface ionization (SI) offers selective ionization of organic nitrogen compounds, making it suitable for breath analysis with ion mobility spectrometry (IMS).

Purpose of the Study:

  • To develop and evaluate a novel stepwise surface ionization (SI) method for detecting organic nitrogen compounds in exhaled breath.
  • To assess the performance of the SI-IMS system for trimethylamine (TMA) detection under varying concentrations and humidity levels.
  • To establish the feasibility of SI-IMS as a sensitive and robust technique for breath analysis in disease diagnostics.

Main Methods:

  • A stepwise SI method involving low-temperature adsorption and high-temperature ionization was developed.
  • Trimethylamine (TMA) was used as a target analyte and detected using an ion mobility spectrometer coupled with the SI system.
  • The method's performance was evaluated by analyzing TMA at various concentrations and humidity levels, including spiking in human breath.

Main Results:

  • The SI-IMS method successfully detected TMA in concentrations ranging from 2 to 200 ppb.
  • A strong linear correlation (R²=0.996) was observed between TMA concentration and peak ion intensity.
  • The method demonstrated excellent repeatability (standard deviation <0.306%), minimal signal variation in high humidity (>93% RH, 2.7%), and high recovery rates (106.8%).

Conclusions:

  • The developed stepwise SI-IMS method is characterized by low ionization temperature, high sensitivity, and robust resistance to humidity.
  • The technique exhibits excellent repeatability, making it reliable for quantitative analysis.
  • SI-IMS is a promising approach for the sensitive and specific detection of organic nitrogen compounds in exhaled breath for early disease diagnosis.