Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

11.6K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Authors' response.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2026
Same author

Patterns of Chronic Stress in Diurnal Salivary Cortisol Already Predict Epigenetic Age Acceleration in Young Adults in the Cebu Longitudinal Health and Nutrition Survey.

American journal of human biology : the official journal of the Human Biology Council·2026
Same author

Social determinants of health and epigenetic clocks: a systematic review and meta-analysis of 140 studies.

Nature human behaviour·2026
Same author

Immigrant Generation and Depressive Symptom Trajectories from Early Adolescence to Midlife: Evidence from Add Health.

Journal of health and social behavior·2026
Same author

A Lasting Legacy: Long-Term Effects of Exercise Training on Cardiometabolic Health in the STRRIDE-Prediabetes Reunion Study.

medRxiv : the preprint server for health sciences·2026
Same author

A Reproducible Pipeline for Processing Commercial Wearable Step-Count Data in Aging Cohorts: Application and Evaluation in the STRRIDE-PD Reunion Study.

medRxiv : the preprint server for health sciences·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: May 2, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

25.5K

Accounting for differences between Infinium MethylationEPIC v2 and v1 in DNA methylation-based tools.

Beryl C Zhuang1,2,3, Marcia Smiti Jude1,2,3, Chaini Konwar1,2,3

  • 1Edwin S. H. Leong Centre for Healthy Aging, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.

Biorxiv : the Preprint Server for Biology
|July 15, 2024
PubMed
Summary
This summary is machine-generated.

The new Illumina MethylationEPIC BeadChip Infinium microarray v2.0 (EPICv2) shows high array-level correlation but variable probe-level concordance with EPICv1. Version differences impact DNA methylation tools, necessitating careful harmonization in research.

Keywords:
DNA methylationEpigeneticsIllumina EPIC arraycell type deconvolutionepigenetic clocksinflammation and lifestyle biomarkers

More Related Videos

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

5.5K
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

4.5K

Related Experiment Videos

Last Updated: May 2, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

25.5K
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

5.5K
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

4.5K

Area of Science:

  • Epigenetics
  • Genomics
  • Biotechnology

Background:

  • The Illumina MethylationEPIC BeadChip Infinium microarray v2.0 (EPICv2) is a new DNA methylation profiling platform.
  • Its concordance with the previous version (EPICv1) is high at the array level but has not been fully assessed for downstream DNA methylation-based tools.

Purpose of the Study:

  • To investigate the concordance between EPICv1 and EPICv2 for DNA methylation-based tools, including cell type deconvolution, epigenetic clocks, and biomarkers.
  • To evaluate the impact of EPIC version differences on these tools and explore mitigation strategies.

Main Methods:

  • DNA methylation was profiled using both EPICv1 and EPICv2 on matched venous blood samples from four adult cohorts.
  • Data analysis focused on array-level and probe-level concordance, and the impact of version differences on various DNA methylation-based tools.
  • Normalization methods and version adjustment strategies were assessed.

Main Results:

  • Samples clustered by EPIC version, indicating systematic differences between the platforms.
  • High array-level concordance was observed, but probe-level concordance varied significantly.
  • Estimates from DNA methylation-based tools showed significant version-specific differences, even after normalization.

Conclusions:

  • Accounting for EPIC version is crucial for research, particularly in meta-analyses and longitudinal studies involving samples profiled on different versions.
  • Version differences can impact epigenome-wide association studies (EWAS) findings, especially for poorly concordant probes.
  • Adjusting for EPIC version or analyzing data separately for each version can mitigate discordances.