Related Experiment Video
Updated: Jun 21, 2025
09:22
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
25.0K
A spatially resolved multiomic single-cell atlas of soybean development
Xuan Zhang1,2, Ziliang Luo1,2, Alexandre P Marand3,2
1Department of Genetics, University of Georgia, Athens, GA, USA.
Biorxiv : the Preprint Server for Biology
|July 15, 2024
Summary
This study maps gene regulation across soybean tissues, revealing cell-specific regulatory elements and transcription factors. It provides a foundation for understanding gene control in diverse soybean cell types.
Area of Science:
- Plant molecular biology
- Genomics
- Cell biology
Background:
- Cis-regulatory elements (CREs) are crucial for precise gene expression control.
- Understanding CREs is key to deciphering cell identity and function.
Purpose of the Study:
- To create a spatially resolved single-cell atlas of gene expression and chromatin accessibility in soybean.
- To identify cell-type-specific CREs and transcription factor (TF) motifs.
- To explore gene regulatory networks in soybean development and symbiosis.
Main Methods:
- Single-cell RNA sequencing and ATAC-seq across ten soybean tissues.
- Identification of cell types, accessible chromatin regions (ACRs), and TF motifs.
- Analysis of developmental trajectories and conservation of regulatory networks.
Main Results:
- Identified 103 distinct soybean cell types and 303,199 ACRs.
- Discovered that nearly 40% of ACRs are cell-type-specific and enriched for defining TF motifs.
- Uncovered regulatory mechanisms in endosperm development, sucrose transport, and embryo cell specification, including a homeobox TF for cotyledon identity.
Conclusions:
- This atlas provides a comprehensive resource for studying gene regulation in soybean.
- Identified novel CREs and TFs critical for soybean cell identity and development.
- Offers insights into the genetic basis of legume symbiotic nitrogen fixation and crop improvement.
Related Concept Videos
02:16Light Acquisition
8.4K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.4K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K