Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

ROOT-ExM: Super-Resolution Imaging of Proteins in <i>Arabidopsis</i> Roots by Expansion Microscopy.

Bio-protocol·2026

Same author

A dual phospholipase system instructs membrane hydrolysis during the final stages of plant autophagy.

Nature communications·2026

Same author

Publisher Correction: Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity.

Nature plants·2026

Same author

Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity.

Nature plants·2026

Same author

Variable temperature processing by plasmodesmata regulates robust bud dormancy release.

Nature communications·2026

Same author

Stretching the boundaries: Expansion microscopy a game changer in super-resolution imaging.

Current opinion in plant biology·2026

Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026

Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026

Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026

Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026

Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026

Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 21, 2025

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.

Ziqiang P Li^1,2, Jennifer Huard¹, Emmanuelle M Bayer¹

¹UMR 5200 Laboratoire de Biogenèse Membranaire, CNRS-University of Bordeaux, Villenave d'Ornon, France.

|July 15, 2024

Summary

This summary is machine-generated.

This study presents a new CRISPR-Cas9 method for efficient, multiplexed plant genome editing. The versatile strategy allows simultaneous targeting of multiple genes with synchronized Cas9 expression, enabling precise genetic modifications in Arabidopsis.

Keywords:

Arabidopsis thaliana CRISPR-Cas9 Gateway® cloning Golden Gate cloning Plant genome editing sgRNA multiplexing

More Related Videos

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Published on: October 17, 2017

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Published on: October 4, 2021

Related Experiment Videos

Last Updated: Jun 21, 2025

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Published on: October 17, 2017

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Published on: October 4, 2021

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

CRISPR-Cas9 is a key tool for plant genome editing.
Advancements allow simultaneous multi-gene targeting and inducible/tissue-specific editing.
Tailored genome modifications are increasingly possible.

Purpose of the Study:

To develop an optimized, modular CRISPR-Cas9 strategy for simultaneous multi-gene targeting.
To enable synchronized Cas9 expression in specific plant cells (e.g., egg cells).
To provide a versatile system adaptable for inducible or tissue-specific editing.

Main Methods:

Developed a cloning strategy combining Gateway® and Golden Gate technologies.
Created a modular vector system for multiplexed single-guide RNA (sgRNA) expression.
Assembled constructs using Multisite Gateway® Technology for Cas9 expression and sgRNA targeting.
Utilized egg cell-specific promoters for synchronized Cas9 expression.

Main Results:

Achieved high editing efficiency while avoiding mosaicism.
Demonstrated simultaneous targeting of multiple genes (e.g., MCTP3 and MCTP4 in Arabidopsis).
Successfully combined multiplexed sgRNA expression with synchronized Cas9 delivery.

Conclusions:

The developed system offers a versatile and efficient method for plant genome editing.
The modular approach simplifies the generation of multi-gene edited lines.
This strategy facilitates precise, tailored genetic modifications in plants.