Integrated magneto-plasmonic nanostructures-based immunoassay for galectin-3 detection.
Sara Fateixa1, Ana L F Martins1, Beatriz Colaço1
1CICECO-Aveiro Institute of Materials and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. sarafateixa@ua.pt.
Analytical Methods : Advancing Methods and Applications
|July 15, 2024
Summary
This study introduces a novel surface-enhanced Raman scattering (SERS) method for detecting galectin-3 (Gal-3), a key cardiovascular disease biomarker. The developed nanoplatform offers sensitive and specific detection, paving the way for improved cardiovascular diagnostics.
Area of Science:
- Nanotechnology
- Spectroscopy
- Biomedical Diagnostics
Background:
- Cardiovascular diseases are a major global health concern, necessitating advanced diagnostic tools.
- Galectin-3 (Gal-3) is a critical biomarker for cardiovascular conditions, but its detection requires sensitive methods.
- Surface-enhanced Raman scattering (SERS) spectroscopy shows promise for biomarker detection, yet its application for Gal-3 is unexplored.
Purpose of the Study:
- To develop novel plasmonic and magneto-plasmonic nanoplatforms for sensitive immunodetection of Gal-3 using SERS.
- To establish SERS methodology for Gal-3 detection, a biomarker previously unaddressed by this technique.
- To evaluate the sensitivity, specificity, and potential clinical applicability of the developed nanoplatforms.
Main Methods:
- Synthesis and functionalization of gold nanoparticles (AuNPs) with specific ligands for antibody conjugation.
- Development of SERS-based immunosensors for Gal-3 detection in buffer solutions.
- Creation of magneto-plasmonic nanoparticles for enhanced Gal-3 uptake and SERS detection coupled with Raman imaging.
Main Results:
- Achieved a limit of detection (LOD) of 12.2 ng mL⁻¹ for Gal-3 with a working range up to 120 ng mL⁻¹.
- Demonstrated high selectivity for Gal-3 in the presence of other common biomolecules.
- Successfully developed and tested magneto-plasmonic nanoparticles for improved Gal-3 detection via SERS and Raman imaging.
Conclusions:
- The developed SERS-based nanoplatforms enable sensitive and specific immunodetection of Gal-3.
- This approach holds significant potential for improving the diagnosis and surveillance of cardiovascular diseases.
- Further optimization and clinical translation of these nanoplatforms are warranted for real-world applications.
