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Areca nut-induced AREG promote oral epithelial cell proliferation, migration, and EMT

  • 0Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University & Hunan Key Laboratory of Oral Health Research, Changsha, China.
Oral Diseases +

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July 15, 2024

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July 15, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Areca nut extract (ANE) affects human oral keratinocyte (HOK) cells by promoting proliferation, migration, and epithelial-mesenchymal transition (EMT) via the AREG-MAPK pathway.

Area Of Science

  • Oral biology
  • Cellular mechanisms
  • Molecular signaling

Background

  • Areca nut extract (ANE) is a common substance with known oral health implications.
  • Understanding its specific effects on human oral keratinocyte (HOK) cells is crucial for assessing oral cancer risk.
  • The molecular pathways involved in ANE's action on HOK cells require further elucidation.

Purpose Of The Study

  • To investigate the biological effects of ANE on HOK cells.
  • To elucidate the underlying molecular mechanisms, focusing on gene and protein expression.
  • To determine the role of the AREG-MAPK signaling pathway in ANE-induced cellular changes.

Main Methods

  • Cell counting kit-8 (CCK-8) assays for proliferation.
  • Second-generation transcriptome sequencing for gene expression analysis.
  • Real-time PCR, Western blot, wound healing, and Transwell assays for gene/protein expression and cell migration.
  • AREG gene overexpression and knockdown experiments.

Main Results

  • ANE demonstrated dose-dependent effects on HOK cell proliferation, with promotion at 500 μg/mL and inhibition at 50 mg/mL.
  • ANE (2 mg/mL) promoted epithelial-mesenchymal transition (EMT) in HOK cells.
  • ANE upregulated AREG protein expression, activating the MAPK signaling pathway and enhancing HOK cell proliferation and migration.

Conclusions

  • ANE promotes HOK cell proliferation, migration, and EMT.
  • The AREG-MAPK signaling pathway is a key mediator of ANE's effects on HOK cells.
  • These findings contribute to understanding the pathogenesis of oral potentially malignant disorders associated with areca nut use.

Keywords:

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