[The experimental uterus autotrasplantation sheep model]

Introduction: A novel method for the treatment of absolute uterine factor infertility is uterine transplantation. Objective: For the sake of team preparation for human uterine transplantation, we carried out experimental sheep animal surgeries in a prospective manner. The aim of the surgeries was the identification of the anatomical structures, the preparation of the graft, the completion of the anastomosis between the vessels, and the team practice of the specialists. Method: The feeding vessels of the uterus were dissected all the way of their origin, followed by hysterectomy with long vascular pedicles. The specimen was perfused with natrium-heparin during the one-hour long cold ischemia, then it was auto-transplanted into the ipsilateral side, after cervico-vaginal anastomoses and end-to-side vascular anastomoses to the anterior surface of the external iliac vessels. Results: In 2 of the 4 cases, the operation has been declared successful, due to the re-occurrence of color, temperature and circulation. Following the investigation, all animals were euthanized. Discussion: Uterine transplantation is a novel method of the treatment of absolute uterine factor infertility. Appropriate preparation and practice on large animal is necessary for the start of a programme. The experimental sheep model provides excellent opportunity to prepare for both live and dead donor human operations. Conclusion: We conclude that the experimental sheep model is a reliable method for the preparation for human uterus transplantation trials. Orv Hetil. 2024; 165(28): 1079–1085.