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Rab10-CAV1 mediated intraluminal vesicle transport to migrasomes

Yong Li1,2, Yiling Wen2, Ying Li3

  • 1Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100084, China.

Proceedings of the National Academy of Sciences of the United States of America
|July 15, 2024
PubMed

Insights

Researchers discovered how intraluminal vesicles, marked by Rab10 and Caveolin-1 (CAV1), are transported to migrasomes. This mechanism, involving Myosin Va, RILPL2, and LRRK2-mediated Rab10 phosphorylation, is vital for cell communication and wound healing.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Migrasomes are key organelles in cell migration and intercellular communication.
  • Intraluminal vesicles within migrasomes are crucial cargoes, but their transport mechanism is unknown.
  • Understanding migrasome cargo transport is essential for deciphering cell signaling pathways.

Purpose of the Study:

  • To elucidate the mechanism of intraluminal vesicle transport to migrasomes.
  • To identify the molecular players involved in this transport process.
  • To demonstrate the physiological relevance of this transport in wound healing.

Main Methods:

  • Utilized immunofluorescence microscopy to visualize Rab10 and Caveolin-1 (CAV1) in migrasomes.
  • Employed biochemical assays to study the roles of Myosin Va, RILPL2, and LRRK2.
  • Investigated the effect of CSF-1 transport via migrasomes on monocyte-macrophage differentiation.

Main Results:

  • Identified Rab10 and CAV1 as markers for intraluminal vesicles within migrasomes.
  • Demonstrated that Myosin Va and RILPL2 are essential for transporting these vesicles.
  • Showed that LRRK2-mediated phosphorylation of Rab10 regulates the transport process.
  • Confirmed CSF-1 transport to migrasomes, promoting monocyte-macrophage differentiation.

Conclusions:

  • A novel mechanism for transporting Rab10-CAV1 vesicles to migrasomes has been identified.
  • This pathway involves Myosin Va, RILPL2, and LRRK2-regulated Rab10.
  • The transport of CSF-1 via migrasomes plays a significant role in skin wound healing and immune cell differentiation.
Keywords:
Caveolin 1Rab GTPasesmigrasomevesicle trafficking

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