Sulfadiazine degradation by Bjerkandera adusta DH0817 at low temperatures and its cold-adaptation mechanisms

The prolonged period of low temperatures in northern China poses a significant challenge to the bioremediation of antibiotic pollution. This study reports that a white-rot fungus Bjerkandera adusta DH0817, isolated from a poultry farm in Liaoning Province, can remove 60 % of SDZ within 20 days at 10°C and reduce the biotoxicity of SDZ. Six degradation pathways were proposed. SDZ biodegradation was primarily driven by cytochrome P450. Transcriptome analysis revealed that DH0817 upregulated genes associated with cell membrane, transcription factors and soluble sugars in response to low temperatures. Subsequently, genes associated with fatty acid, proteins and enzymes were upregulated to remove SDZ at low temperatures. This study provides valuable microbial resources and serves as a theoretical reference for addressing antibiotic pollution in livestock and poultry farms under low temperature conditions.