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Microbial determinations by flow cytometry.

K J Hutter, H E Eipel

    Journal of General Microbiology
    |August 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Flow cytometry advancements enable precise measurement of microbial DNA and protein content. This technique accurately determines ploidy and analyzes large cell populations rapidly.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biotechnology

    Background:

    • Recent advancements in flow cytometry optics, electronics, and staining techniques have enhanced its capability.
    • These improvements allow for the precise measurement of minute cellular components like DNA and protein.

    Purpose of the Study:

    • To assess the utility of advanced flow cytometry for analyzing microbial cellular constituents.
    • To determine DNA and protein content distributions in various microorganisms.
    • To evaluate the method's effectiveness in ploidy determination.

    Main Methods:

    • Flow cytometry was employed to measure DNA and protein content.
    • Distributions of DNA and protein content were analyzed in Escherichia coli, Lactobacillus species, Chlorella kessleri, Saccharomyces cerevisiae, Candida utilis, Schizosaccharomyces pombe, and Euglena gracilis.

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  • Polyploid strains of Saccharomyces cerevisiae were specifically investigated for ploidy assessment.

    • Main Results:

    • DNA and protein content distributions were successfully determined for all tested microorganisms.
    • Flow cytometry accurately identified and quantified DNA content in polyploid Saccharomyces cerevisiae strains, confirming its utility for ploidy determination.
    • The rapid nature of flow cytometry measurements enabled accurate analysis of large microbial populations.

    Conclusions:

    • Advanced flow cytometry is a valuable and accurate technique for quantifying microbial DNA and protein content.
    • The method is effective for determining microbial ploidy and analyzing large cell populations.
    • This technique offers a rapid and precise approach for microbial cellular analysis.