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Optimized Partial Freezing Protocol Enables 10-Day Storage of Rat Livers
Ozge Sila Ozgur1, McLean S Taggart1, Mohammedreza Mojoudi1
1Massachusetts General Hospital, Harvard Medical School.
Research Square
|July 16, 2024
Summary
Researchers optimized partial freezing to preserve rodent livers for 10 days, significantly extending viability beyond current methods. This advance in organ preservation could expand donor organ availability for transplantation.
Area of Science:
- Organ preservation
- Transplant medicine
- Cryobiology
Background:
- Organ preservation is limited by time, impacting donor organ availability.
- Partial freezing at -15°C previously enabled 5-day rodent liver storage.
- Limitations included increased vascular resistance and tissue edema.
Purpose of the Study:
- To optimize the partial freezing protocol for extended liver preservation.
- To improve liver viability and function post-preservation.
- To enhance the potential for organ transplantation.
Main Methods:
- Optimized partial freezing protocol using increased propylene glycol concentration.
- Maintained osmotic balance with enhanced bovine serum albumin concentration.
- Minimized cryoprotectant unloading stress via an acclimation period.
Main Results:
- Achieved 10-day liver preservation, outperforming standard cold storage.
- Demonstrated comparable vascular resistance and lactate levels to 5-day preservation.
- Observed improved liver function, evidenced by increased oxygen consumption and reduced edema.
Conclusions:
- Optimized partial freezing significantly extends viable organ preservation time.
- This method offers a promising alternative to current organ storage techniques.
- Advancements in organ preservation can revolutionize transplant medicine by expanding donor organ availability.
