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Updated: May 8, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Back to the future: Using herbarium specimens to isolate nodule-associated bacteria.

Renee H Petipas¹, Amanda A Antoch^1,2, Ashton A Eaker¹

¹Department of Plant Pathology Washington State University Pullman Washington USA.

Ecology and Evolution

|July 16, 2024

Summary

Historic bacteria were successfully cultured from herbarium specimens of Medicago lupulina nodules. This breakthrough enables new research into plant-microbe interactions across time.

Keywords:

Medicago lupulina coevolution herbarium collections nodule microbiome rhizobia

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Area of Science:

Microbiology
Botany
Evolutionary Biology

Background:

Herbarium specimens are valuable resources for studying plant ecology and evolution.
Current research primarily uses specimens for genetic material via culture-independent methods.

Purpose of the Study:

To demonstrate the feasibility of culturing nodule-associated bacteria from herbarium specimens.
To explore plant-microbe interactions at novel spatiotemporal scales using historic samples.

Main Methods:

Historic and contemporary nodules from Medicago lupulina were used to establish a culture collection.
Bacteria were isolated and identified from specimens collected in 1950, 2004, and 2015.

Main Results:

Historic bacteria from 15 genera were successfully recovered from herbarium specimens.
This is the first study to isolate historic bacteria from herbarium samples.
While the primary symbiont Ensifer was not recovered, other potential nodulating and growth-promoting bacteria were identified.

Conclusions:

Herbarium specimens offer a viable method for culturing historic bacteria, expanding research possibilities for plant-microbe interactions.
Future studies should involve inoculating plants with historic strains to assess nodulation, plant phenotype, and fitness.