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Published on: December 5, 2020
Dactylfungins and Tetralones: Bioactive Metabolites from a Nematode-Associated Laburnicola nematophila
Jan-Peer Wennrich1,2, Caren Holzenkamp1,2, Miroslav Kolařík3
1Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Abstract:
A chemical investigation of Laburnicola nematophila, isolated from cysts of the plant parasitic nematode Heterodera filipjevi, affored three dactylfungin derivatives (1-3) and three tetralone congeners (4-6). Dactylfungin C (1), laburnicolin (4), and laburnicolenone (5) are previously undescribed natural products. Chemical structures of the isolated compounds were determined based on 1D and 2D NMR spectroscopic analyses together with HR-ESI-MS spectrometry and comparison with data reported in the literature. The relative configurations of compounds 1, 2, and 4-6 were determined based on their ROESY data and analysis of their coupling constants (J values). The absolute configurations of 4-6 were determined through the comparison of their measured and calculated TDDFT-ECD spectra. Compounds 1-3 were active against azole-resistant Aspergillus fumigatus.
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