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Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
06:04

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

Effect of parental aspirin on the gastric mucosal barrier in the rat

P H Guth, G Paulsen

    Digestion
    |January 1, 1979
    PubMed

    Insights

    Parenteral aspirin causes gastric mucosal lesions in rats. Lesions appear before significant disruption of the gastric mucosal barrier, suggesting barrier disruption is a consequence, not the cause, of aspirin-induced injury.

    Area of Science:

    • Gastroenterology
    • Pharmacology
    • Toxicology

    Background:

    • Aspirin is known to cause gastric mucosal injury.
    • The mechanism involves disruption of the gastric mucosal barrier to hydrogen ion back-diffusion.

    Purpose of the Study:

    • To investigate if parenteral aspirin injures the gastric mucosa by disrupting the gastric mucosal barrier.
    • To determine the temporal relationship between lesion formation and barrier disruption.

    Main Methods:

    • Rats were administered sodium acetylsalicylate intraperitoneally.
    • Gastric mucosal lesions were scored.
    • Back-diffusion studies, including ion fluxes and lumenal potential difference, were conducted at 0.5 and 4 hours post-administration.

    Main Results:

    • Gastric mucosal lesions developed by 0.5 hours after aspirin administration.
    • The gastric mucosal barrier remained intact at 0.5 hours.
    • By 4 hours, lesions were more severe, accompanied by a fall in potential difference and increased hydrogen ion loss, indicating barrier disruption.

    Conclusions:

    • In this rat model, gastric mucosal lesions occur before evidence of diffuse barrier disruption.
    • Diffuse barrier disruption is a consequence, not the initial cause, of aspirin-induced gastric mucosal injury.

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