Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Predicting human mRNA isoform levels from site-specific splicing kinetics <i>in silico</i>.

bioRxiv : the preprint server for biology·2026

Same author

Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.

APL photonics·2026

Same author

PRT-based multisensory smart education prototype for intervention in children with autism spectrum disorder.

iScience·2026

Same author

Weight-Bearing Test: A Straightforward and Reliable Indicator of Stability in Supination-External Rotation Type, Weber B Ankle Fractures.

Clinics in orthopedic surgery·2026

Same author

Fatty acid biosynthesis inhibitors fabimycin and triclosan trigger distinct resistance mutations in FabI and potently kill Gram-negative pathogens.

bioRxiv : the preprint server for biology·2026

Same author

Heterogenous Model of Temozolomide Resistance in Glioblastoma Reveals Phenotypic Shifts in Drug Response and Migratory Potential.

Advanced nanobiomed research·2026

Search research articles

Related Experiment Video

Updated: Jun 20, 2025

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Capturing cell morphology dynamics with high temporal resolution using single-shot quantitative phase gradient

Sun Woong Hur^1,2, Minsung Kwon^1,2, Revathi Manoharaan^1,2

¹University of Illinois at Urbana-Champaign, Department of Bioengineering, Urbana, Illinois, United States.

Journal of Biomedical Optics

|July 17, 2024

Summary

Fast, label-free quantitative phase imaging (ss-QPGM) monitors cellular changes in real-time. This technique reveals morphological and biochemical shifts during cell death, offering a promising tool for live-cell dynamics.

Keywords:

cell death label-free imaging morphology quantitative phase imaging single-shot imaging

More Related Videos

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Related Experiment Videos

Last Updated: Jun 20, 2025

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Area of Science:

Biomedical Optics
Cell Biology
Microscopy

Background:

Label-free quantitative phase imaging (QPI) offers non-perturbing measurement of cellular dynamics.
Developing faster and more sensitive instrumentation is crucial for capturing rapid cellular events.
Single-shot quantitative phase gradient microscopy (ss-QPGM) enables simultaneous acquisition of polarization components for phase reconstruction.

Purpose of the Study:

To characterize a fast, single-shot quantitative phase gradient microscopy (ss-QPGM) approach.
To record morphological changes in single-cell phase images.
To correlate these morphological changes with biochemical indicators of cell death using fluorescence imaging.

Main Methods:

Implemented a computationally efficient least squares algorithm for real-time, video-rate imaging (up to 30 fps).
Utilized ss-QPGM to acquire phase images and concurrently acquired fluorescence images of biochemically labeled cells.
Validated phase measurement accuracy using a USAF1951 pattern phase target and calibrated sample thicknesses.

Main Results:

Achieved a spatial resolution of 0.8 µm and accurate phase retrieval (R² > 0.99).
Demonstrated real-time, video-rate (30 fps) ss-QPGM for live-cell imaging.
Observed dynamic intracellular and morphological changes during nutrient deprivation and drug-induced cell death, correlating with necrosis.

Conclusions:

Label-free ss-QPGM provides high-temporal resolution and high spatial fidelity for live-cell imaging.
The technique effectively monitors dynamic cellular changes during induced cell death.
ss-QPGM shows significant promise for studying live-cell dynamics with minimal perturbation.