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Related Experiment Video

Updated: Jun 20, 2025

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Published on: August 18, 2017

Energy-Resolved In-Source Collison-Induced Dissociation for Isomer Discrimination.

Matthew J Carlo¹, Andie L M Nanney¹, Amanda L Patrick¹

¹Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.

Journal of the American Society for Mass Spectrometry

|July 17, 2024

Summary

In-source collision-induced dissociation (IS-CID) offers a way to differentiate isomers using energy-resolved measurements, even on simpler mass spectrometers. This method enhances selectivity without requiring complex additional equipment.

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Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

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Published on: January 20, 2022

Area of Science:

Analytical Chemistry
Mass Spectrometry
Separation Science

Background:

Mass spectrometry is crucial for analyte analysis but struggles with isomer differentiation.
Tandem mass spectrometry and energy-resolved measurements improve selectivity but require specialized instruments.
Compact and affordable mass spectrometers often lack capabilities for advanced dissociation experiments.

Purpose of the Study:

To investigate energy-resolved in-source collision-induced dissociation (IS-CID) as a method for isomer differentiation.
To compare energy-resolved appearance curves from IS-CID with those from traditional tandem mass spectrometry.
To evaluate IS-CID's potential for differentiating isomers with similar dissociation patterns and explore implementation considerations.

Main Methods:

Utilized in-source collision-induced dissociation (IS-CID) on single-stage mass spectrometers.
Measured energy-resolved appearance curves using IS-CID.
Compared IS-CID results with data from a quadrupole time-of-flight instrument employing true tandem mass spectrometry.
Assessed the ability of IS-CID to differentiate isomers and isobar sets.

Main Results:

Energy-resolved appearance curves obtained via IS-CID show analytical potential comparable to tandem mass spectrometry.
IS-CID effectively differentiates isomers and isobar sets, including those with similar fragmentation patterns.
The study explored analytical considerations for method development and implementation of IS-CID.

Conclusions:

Energy-resolved IS-CID provides a viable alternative for isomer differentiation on mass spectrometers lacking advanced capabilities.
This approach broadens the accessibility of enhanced selectivity in mass spectrometry.
IS-CID presents a promising technique for future method development in various applications, including field-portable devices and educational settings.