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Changes in ROS/RNS Levels in Endothelial Cells in Experimental Bacteremia
Svetlana N Pleskova1,2, Alexander N Vaneev3,4, Nikolay A Bezrukov1
1Research Laboratory of Scanning Probe Microscopy, Lobachevsky State University of Nizhny Novgorod, Gagarina Ave. 23, Build. 3, Nizhny Novgorod, 603950, Russia.
Chembiochem : a European Journal of Chemical Biology
|July 17, 2024
Summary
A new electrochemical system precisely quantifies reactive oxygen species (ROS) and reactive nitrogen species (RNS) in single cells. This system revealed complex cellular interactions in an experimental bacteremia model, highlighting neutrophil roles.
Area of Science:
- Biomedical Engineering
- Cellular Biology
- Analytical Chemistry
Background:
- Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play critical roles in cellular signaling and pathology.
- Quantifying ROS and RNS in single cells during complex biological processes like infection remains challenging.
Purpose of the Study:
- To develop and validate a high-precision electrochemical amperometric system for single-cell ROS and RNS quantification.
- To investigate the dynamics of ROS/RNS production in endothelial cells during experimental bacteremia.
Main Methods:
- Development of a high-precision electrochemical amperometric system for single-cell analyte quantification.
- Evaluation of the system using an experimental bacteremia model involving endothelial cells, Staphylococcus aureus, and neutrophils.
- Quantitative assessment of ROS/RNS levels under various stimulation conditions.
Main Results:
- Endothelial cells showed increased ROS/RNS production upon stimulation with Staphylococcus aureus.
- Sequential stimulation with bacteria followed by neutrophils significantly elevated ROS/RNS levels in endothelial cells.
- A positive correlation was observed between ROS/RNS levels and neutrophil-endothelial cell contact.
Conclusions:
- The developed system offers a powerful tool for quantitative assessment of ROS/RNS dynamics in complex biological systems.
- Findings provide insights into the intricate cellular interplay during experimental bacteremia, particularly the role of neutrophils.
