Multi-modal mass spectrometry imaging of a single tissue section

Area of Science:

• Biomedical Imaging
• Analytical Chemistry
• Molecular Biology

Background:

• Mass spectrometry imaging (MSI) visualizes spatial chemical information in tissues for multi-omic analysis.
• Correlating diverse chemical data within tissues is challenging due to limited spatial information from single MSI modalities.
• Multimodal MSI, visualizing multiple biomolecules simultaneously, is gaining attention.

Purpose of the Study:

• To provide an overview of multimodal MSI on a single tissue section.
• To highlight the application of Matrix-Assisted Laser Desorption/Ionization-MSI for spatial multi-omic investigations.
• To inspire novel approaches in spatial multi-omic analysis.

Main Methods:

• Review of multimodal MSI strategies.
• Focus on single tissue section imaging.
• Emphasis on Matrix-Assisted Laser Desorption/Ionization-MSI.

Main Results:

• Multimodal MSI on a single tissue section integrates diverse chemical data effectively.
• This approach circumvents issues associated with analyzing consecutive tissue sections.
• Matrix-Assisted Laser Desorption/Ionization-MSI facilitates in-depth spatial multi-omic elucidation.

Conclusions:

• Multimodal MSI on single tissue sections is a powerful strategy for spatial multi-omics.
• It offers comprehensive insights into biological states and activities.
• Further development of these techniques promises advanced biological investigations.