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Epigenomic Profiling of B Cell Subsets by CUT&Tag
Bagdeser Akdogan-Ozdilek1, Lou-Ella M M George-Alexander1, Christopher D Scharer2
1Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
Abstract:
Epigenetic programs play a key role in regulating the development and function of immune cells. However, conventional methods for profiling epigenetic mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identifying histone modifications in human and mouse lymphocytes.

