Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

12.7K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
12.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aberrant zonal recycling of germinal center B cells impairs appropriate selection in lupus.

Cell reports·2024
Same author

A novel microporous biomaterial vaccine platform for long-lasting antibody mediated immunity against viral infection.

Journal of controlled release : official journal of the Controlled Release Society·2024
Same author

A novel microporous biomaterial vaccine platform for long-lasting antibody mediated immunity against viral infection.

bioRxiv : the preprint server for biology·2024
Same author

Improved Humoral Immunity and Protection against Influenza Virus Infection with a 3d Porous Biomaterial Vaccine.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2023
Same author

Cutting Edge: IL-21 and Tissue-Specific Signals Instruct Tbet+CD11c+ B Cell Development following Viral Infection.

Journal of immunology (Baltimore, Md. : 1950)·2023
Same author

Development of Tbet- and CD11c-expressing B cells in a viral infection requires T follicular helper cells outside of germinal centers.

Immunity·2022
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2025

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

15.2K

ELISpots for Detecting Antigen-Specific Memory B Cells in Infection or Autoimmunity.

Eden S Hirsch1, Jason S Weinstein2,3

  • 1Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 17, 2024
PubMed
Summary
This summary is machine-generated.

The Enzyme-Linked Immunosorbent Spot assay (ELISpot) quantifies individual protein-secreting cells, offering unmatched single-cell analysis. This modified ELISpot method detects antigen-specific memory B cells in viral infections and autoimmune diseases.

Keywords:
Anti-viral antibodiesAntibody secreting cellAntigen-specific antibodiesAutoantibodies detection

More Related Videos

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
06:13

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

34.8K
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
08:04

Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

Published on: July 9, 2014

15.8K

Related Experiment Videos

Last Updated: Jun 20, 2025

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

15.2K
Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
06:13

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

34.8K
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
08:04

Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

Published on: July 9, 2014

15.8K

Area of Science:

  • Immunology
  • Cellular Biology
  • Assay Development

Background:

  • Enzyme-Linked Immunosorbent Spot assay (ELISpot) is a key immunoassay for quantifying individual protein-secreting cells.
  • ELISpot assays detect secreted proteins at a single-cell level, distinguishing them from population-level measurements like ELISA.
  • The sensitivity and versatility of ELISpots support diverse applications, including cancer research and vaccine development.

Purpose of the Study:

  • To describe a modified ELISpot assay.
  • To demonstrate the assay's utility in detecting antigen-specific memory B cells.
  • To apply the assay in the context of viral infection and autoimmunity.

Main Methods:

  • Utilized a modified Enzyme-Linked Immunosorbent Spot assay (ELISpot).
  • Focused on detecting antigen-specific memory B cells.
  • Applied the assay in models of viral infection and autoimmunity.

Main Results:

  • The modified ELISpot assay successfully detected antigen-specific memory B cells.
  • The study demonstrated the assay's effectiveness in relevant disease models.
  • The results highlight the assay's capability for single-cell protein secretion analysis.

Conclusions:

  • The modified ELISpot assay provides a robust method for quantifying antigen-specific memory B cells.
  • This technique offers valuable insights into cellular immune responses in viral infections and autoimmune conditions.
  • The ELISpot assay's ability to evaluate protein secretion at a single-cell level remains a unique advantage.