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  11. Anti-inflammatory Effects And Beneficial Effects Of The Feed Additive Urtica Cannabina L. In Zebrafish

Anti-inflammatory effects and beneficial effects of the feed additive Urtica cannabina L. in zebrafish

Wuyun Liu1,2,3,4, Huarong Yu1,2, D Gurbazar3

  • 1Key Laboratory of Ecological Agriculture in Horqin Sandy Land, State Ethnic Affairs Commission, Wuhan, China.

Plos One
|July 17, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Urtica cannabina L. (UL) exhibits anti-inflammatory properties, effectively reducing inflammation in zebrafish models. UL supplementation also reversed negative effects of LPS and improved gut microbiota balance, suggesting its potential as a feed additive for livestock.

Area of Science:

  • Pharmacology and Toxicology
  • Zebrafish Models in Inflammation Research
  • Gut Microbiome Analysis

Background:

  • Urtica cannabina L. (UL) has a long history of clinical use attributed to its anti-inflammatory properties.
  • Investigating the precise mechanisms and efficacy of UL at various concentrations is crucial for its therapeutic and additive applications.
  • Zebrafish serve as a valuable model organism for studying inflammation due to their physiological similarities to mammals and amenability to experimental manipulation.

Purpose of the Study:

  • To investigate the anti-inflammatory mechanisms and effects of different Urtica cannabina L. (UL) concentrations in zebrafish.
  • To evaluate UL's efficacy in two distinct zebrafish inflammation models: caudal fin resection and lipopolysaccharide (LPS)-induced intestinal inflammation.
  • To assess UL's impact on gut microbiota composition in the context of LPS-induced inflammation.

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Main Methods:

  • Two zebrafish inflammation models were established: caudal fin transection for tissue repair assessment and LPS administration for intestinal inflammation induction.
  • Effects of varying UL concentrations (10 mg/g and 30 mg/g) were evaluated on tail inflammation, feed intake, body weight, intestinal histology (goblet cells), and pro-inflammatory cytokine gene expression (TNF-α, IL6, IL8).
  • Gut microbiota composition was analyzed using 16S rRNA sequencing to determine changes in bacterial phyla abundance.

Main Results:

  • UL supplementation (10 mg/g) alleviated inflammation and promoted healing following caudal fin resection, although higher doses (30 mg/g) did not show the same effect.
  • UL reversed LPS-induced decreases in feed intake, body weight, and intestinal goblet cell counts, while also downregulating pro-inflammatory cytokine gene expression.
  • UL treatment modulated gut microbiota, increasing beneficial Bacteroidota and decreasing pathogenic bacteria in LPS-challenged zebrafish.

Conclusions:

  • Urtica cannabina L. demonstrates significant anti-inflammatory and protective effects in zebrafish models of both physical injury and LPS-induced inflammation.
  • UL positively influences gut health by restoring intestinal integrity and rebalancing the gut microbiota.
  • These findings support the development of Urtica cannabina L. as a feed additive to enhance inflammatory resistance in livestock.