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Related Experiment Videos

Bovine neutrophils treated with chemotactic agents: morphologic changes.

J H Forsell, J R Kateley, C W Smith

    American Journal of Veterinary Research
    |September 1, 1985
    PubMed
    Summary

    Bovine neutrophils rapidly change to a bipolar shape when exposed to immune stimulants like zymosan-activated serum. This shape change serves as a quick in vitro test for early neutrophil chemotaxis.

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    Area of Science:

    • Veterinary Immunology
    • Cell Biology
    • Chemotaxis Research

    Background:

    • Neutrophils are critical immune cells in cattle.
    • Understanding neutrophil behavior is key to diagnosing and treating infections.
    • Early detection of neutrophil chemotaxis is vital for immune response assessment.

    Purpose of the Study:

    • To investigate the rapid shape-change response of bovine neutrophils.
    • To establish a novel in vitro assay for evaluating early chemotactic events in neutrophils.
    • To identify stimuli that induce neutrophil shape changes.

    Main Methods:

    • Isolation of neutrophils from peripheral blood of cattle.
    • Incubation of neutrophils with zymosan-activated serum, bacterial cell filtrate, and casein.

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  • Microscopic observation of neutrophil morphology changes (spherical to bipolar shape).
  • Time-course analysis of shape change induction.

    • Main Results:

    • Neutrophils exhibited a rapid transition from spherical to bipolar shape upon incubation with zymosan-activated serum.
    • Ninety percent of neutrophils displayed a bipolar shape within 5 to 10 minutes.
    • Bacterial cell filtrate and casein also induced neutrophil bipolar shape changes.
    • N-formyl-L-methionyl-L-leucinyl-L-phenylalanine did not induce significant shape changes.

    Conclusions:

    • The neutrophil shape-change response is a rapid and sensitive indicator of early chemotactic events.
    • This assay provides a valuable tool for assessing bovine neutrophil function in vitro.
    • The findings contribute to understanding neutrophil activation pathways and developing diagnostic tools for cattle immunology.