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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
Published on: April 18, 2025
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miR-129-2-3p binds SEMA4C to regulate HCC development and inhibit the EMT
Mutation Research
|July 17, 2024
Summary
MicroRNA-129-2-3p inhibits hepatocellular carcinoma (HCC) by targeting SEMA4C. This finding reveals a new therapeutic target for HCC treatment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Hepatocellular carcinoma (HCC) is the most common primary liver cancer.
- MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression and are involved in various biological processes, including cancer.
Purpose of the Study:
- To investigate the role of miR-129-2-3p in HCC.
- To explore the relationship between miR-129-2-3p and SEMA4C in HCC.
Main Methods:
- Quantitative real-time PCR (RT-qPCR) for miRNA and mRNA expression.
- Cell proliferation, invasion, and migration assays (CCK-8, wound healing, transwell).
- Western blotting (WB), ELISA, RNA immunoprecipitation (RIP), and dual-luciferase reporter assays.
Main Results:
- miR-129-2-3p expression was downregulated in HCC tissues and cells.
- miR-129-2-3p overexpression suppressed HCC cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT).
- SEMA4C was upregulated in HCC and its knockdown inhibited HCC progression; SEMA4C overexpression reversed the effects of miR-129-2-3p.
Conclusions:
- miR-129-2-3p acts as a tumor suppressor in HCC.
- miR-129-2-3p inhibits HCC progression by directly targeting SEMA4C.
- This miRNA-mRNA interaction presents a potential therapeutic strategy for HCC.
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