miR-129-2-3p binds SEMA4C to regulate HCC development and inhibit the EMT

Related Experiment Video

Updated: Jun 20, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

378

Siyuan Ma1, Chun Pu1

  • 1School of Laboratory Medicine, Wannan Medical College, China.

Mutation Research
|July 17, 2024
PubMed
Abstract

Insights

MicroRNA-129-2-3p inhibits hepatocellular carcinoma (HCC) by targeting SEMA4C. This finding reveals a new therapeutic target for HCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Hepatocellular carcinoma (HCC) is the most common primary liver cancer.
  • MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression and are involved in various biological processes, including cancer.

Purpose of the Study:

  • To investigate the role of miR-129-2-3p in HCC.
  • To explore the relationship between miR-129-2-3p and SEMA4C in HCC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for miRNA and mRNA expression.
  • Cell proliferation, invasion, and migration assays (CCK-8, wound healing, transwell).
  • Western blotting (WB), ELISA, RNA immunoprecipitation (RIP), and dual-luciferase reporter assays.

Main Results:

  • miR-129-2-3p expression was downregulated in HCC tissues and cells.
  • miR-129-2-3p overexpression suppressed HCC cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT).
  • SEMA4C was upregulated in HCC and its knockdown inhibited HCC progression; SEMA4C overexpression reversed the effects of miR-129-2-3p.

Conclusions:

  • miR-129-2-3p acts as a tumor suppressor in HCC.
  • miR-129-2-3p inhibits HCC progression by directly targeting SEMA4C.
  • This miRNA-mRNA interaction presents a potential therapeutic strategy for HCC.
Keywords:
EMTHCCSEMA4CmiR-129–2-3p

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