Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

33.5K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.5K
Membrane Fluidity01:26

Membrane Fluidity

11.1K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.1K
Types of RNA01:23

Types of RNA

63.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.5K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.1K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.1K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enantiomeric biodistribution, metabolic profile, and toxicity of 3-chloromethcathinone in Wistar rats following acute exposure.

Journal of analytical toxicology·2025
Same author

<i>Pseudomonas mendocina</i> Isolated from <i>Anopheles</i> Midguts has a Greater Potential to Build Thick Biofilms than <i>Serratia marcescens</i>.

ACS omega·2025
Same author

Gilthead sea bream gut bacteriome as a valuable tool for seafood provenance analysis.

Applied and environmental microbiology·2025
Same author

MicroMundo@Oeiras: citizen science promoting antibiotic stewardship, discovery of new antimicrobials, and monitoring of soil resistance.

FEMS microbiology letters·2025
Same author

Enhancing Drought Tolerance in <i>Salicornia ramosissima</i> Through Biofertilization with Marine Plant Growth-Promoting Bacteria (PGPB).

Plants (Basel, Switzerland)·2025
Same author

Exploring the potential of lipid elicitors to enhance plant immunity.

Progress in lipid research·2025

Related Experiment Video

Updated: Jun 20, 2025

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

4.2K

RNase R Affects the Level of Fatty Acid Biosynthesis Transcripts Leading to Changes in membrane Fluidity.

André Filipe Alípio1, Cátia Bárria1, Vânia Pobre1

  • 1Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.

Journal of Molecular Biology
|July 17, 2024
PubMed
Summary

RNase R ribonuclease regulates fatty acid biosynthesis in Streptococcus pneumoniae. Its absence alters membrane composition, increasing susceptibility to environmental stressors like detergents and nisin.

Keywords:
FASII clusterfatty acids biosynthesispneumococcusribonucleasestress resistance

More Related Videos

Preparation, Purification, and Use of Fatty Acid-containing Liposomes
10:43

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

46.8K
Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

8.8K

Related Experiment Videos

Last Updated: Jun 20, 2025

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

4.2K
Preparation, Purification, and Use of Fatty Acid-containing Liposomes
10:43

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

46.8K
Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

8.8K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • RNase R ribonuclease plays a role in Streptococcus pneumoniae biology.
  • Fatty acid biosynthesis is crucial for bacterial membrane integrity and function.

Purpose of the Study:

  • To investigate the role of RNase R in regulating type II fatty acid biosynthesis (FASII) gene expression.
  • To determine the impact of RNase R on membrane fatty acid composition and cellular stress responses.

Main Methods:

  • Gene expression analysis of the FASII cluster in wild-type and RNase R-deficient strains.
  • Analysis of membrane fatty acid composition using lipidomics.
  • Assessing sensitivity to detergent lysis and bacteriocin nisin.

Main Results:

  • Elimination of RNase R led to overexpression of FASII genes.
  • RNase R is involved in the turnover of FASII transcripts.
  • Mutant strains exhibited altered membrane fatty acid profiles with increased unsaturated and long-chain fatty acids.
  • RNase R deficiency resulted in increased susceptibility to lipid peroxidation, detergent lysis, and nisin.

Conclusions:

  • RNase R plays a critical role in regulating fatty acid biosynthesis and membrane composition in S. pneumoniae.
  • Altered membrane fluidity impacts bacterial adaptation and survival under stress conditions.
  • RNase R influences pneumococcal adaptation to environmental challenges through membrane reprogramming.