Related Experiment Video
Updated: Jun 20, 2025
Methods for Detecting Cough and Airway Inflammation in Mice
Published on: August 2, 2024
Advancing cough research: Methodological insights into cough challenge in guinea pig models using double chamber vs
Jana Plevkova1, Janka Jakusova2, Mariana Brozmanova2
1Department of Pathophysiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia; Centre for Medical Education Support, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia.
Objective:
This study compares two methods of citric acid-induced cough in guinea pigs in whole-body plethysmography (WBP) and double chamber plethysmography (DCP) to evaluate their efficacy.
Methods:
Sixteen specific pathogen-free (SPF) and sixteen conventionally-bred (CON) animals were exposed to 0.4 M citric acid aerosol. They underwent cough provocation using both DCP and WBP methods. The number of coughs and latency to the first cough were recorded and analysed using statistical methods to determine significant differences between the two techniques.
Results:
WBP resulted in significantly higher cough counts (WBP vs. DCP: 13±9 vs 2±3 for SPF; 14±8 vs 5±5 for CON; p<0.0001) and shorter latency (WBP vs. DCP: 59±6 s vs 159±14 s for SPF; 77±4 s vs 112±12 s for CON; p<0.0001) compared to DCP in both groups.
Conclusion:
Methodological differences substantially impact cough responses. WBP provides a more reliable and physiologically relevant methodology for cough assessment, suggesting the need for standardized protocols in cough research to enhance translational relevance.
