Using absorbance detection for hs-SV-AUC characterization of adeno-associated virus
Nicholas R Larson1, George M Bou-Assaf1, Thomas M Laue2
1Analytical Development, Biogen, 225 Binney St, Cambridge, MA, 02142, USA.
Analytical Biochemistry
|July 17, 2024
Abstract
None:
Data are presented demonstrating that absorbance detection can be used during high-speed sedimentation velocity analytical ultracentrifugation (hs-SV-AUC) experiments to characterize the size distribution of adeno-associated virus (AAV) drug products accurately. Advantages and limitations of being able to use this detector in this specific type of SV-AUC experiment are discussed.
Keywords:
Adeno-associated virusAnalytical ultracentrifugationHigh rotor speedsHigh-resolutionInterference detectionLow temperatureNanoparticlesSedimentation velocitySize distributionViral vectors
