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STAT transcription factor is indispensable for oogenesis in silkworm.

Shiyuan Wang1, Li Zhou1, Wenjuan Liang1

  • 1Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, China; Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400715, PR China.

International Journal of Biological Macromolecules
|July 17, 2024
PubMed
Summary

The Signal Transducer and Activator of Transcription-Like (STAT-L) gene is vital for silkworm egg development. Knocking out STAT-L causes reproductive issues and egg chamber fusion by affecting Notch signaling.

Keywords:
Bombyx moriOogenesisSTAT

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Signal Transducer and Activator of Transcription (STAT) proteins are crucial transcription factors in eukaryotes.
  • In Drosophila, STAT regulates oogenesis, but its role in silkworms is unknown.

Purpose of the Study:

  • To investigate the function of the STAT-L gene in silkworm egg development.
  • To elucidate the molecular mechanisms by which STAT-L influences oogenesis.

Main Methods:

  • CRISPR/Cas9 gene editing was used to create STAT-L knockout silkworms.
  • Ovarian transcriptomic analysis was performed on STAT-L knockout and control silkworms.
  • Histological analysis of ovaries was conducted.

Main Results:

  • STAT-L knockout silkworms exhibited reproductive abnormalities, including shortened fallopian tubes and reduced egg production.
  • Ovaries from knockout silkworms showed fused egg chambers.
  • Transcriptomic analysis revealed that STAT-L down-regulates APH-1 gene expression, modulating the Notch signaling pathway.

Conclusions:

  • The STAT-L gene is essential for normal egg chamber formation in silkworms.
  • STAT-L plays a critical role in silkworm oogenesis, potentially via the Notch signaling pathway.