Related Experiment Video For ATP
Updated: Jun 20, 2025

In vitro Measurements of Tracheal Constriction Using Mice
Published on: June 25, 2012
ATP Increases Ciliary Beat Frequency and Ciliary Bend Angle through Distinct Purinergic Receptors in Bronchial
Tomoki Sekiya1, Kazuhito Murakami1, Yoichiro Isohama1
1Laboratory of Applied Pharmacology, Faculty of Pharmaceutical Science, Tokyo University of Science.
Abstract:
Airway ciliary cells are components of the mucociliary transport system and play an important role in sweeping out small particles, such as bacteria and viruses, towards the oropharynx by the action of beating cilia. Several lines of evidence have shown that the ciliary beat is under the regulation of the purinergic system; however, the subtype of receptor and the intracellular signaling pathways involved in the activation of ciliary movement remain to be elucidated. In addition, although the activity of ciliary movement comprises two parameters, the ciliary beat frequency (CBF) and ciliary bend angle (CBA), few reports have analyzed CBA. In this study, we examined the effects of ATP and other purinergic ligands on both CBF and CBA and demonstrated that the purinergic signaling requirements for CBF and CBA are different, with CBF mediated by P2Y1 receptor activation and CBA mediated by the P2X4 receptor.
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