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The Hard Life Of An Octopus Embryo Is Seen Through Gene Expression, Energy Metabolism, And Its Ability To Neutralize Radical Oxygen Species.

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The Hard Life Of An Octopus Embryo Is Seen Through Gene Expression, Energy Metabolism, And Its Ability To Neutralize Radical Oxygen Species.

Related Experiment Video

Analysis of Oxidative Stress in Zebrafish Embryos

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

The hard life of an octopus embryo is seen through gene expression, energy metabolism, and its ability to neutralize

Sadot Ramos-Rodríguez¹, Karen Ortega-Ramírez², Luisa Méndez-Can²

¹Laboratorio de Genómica Funcional, Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), CP 22860, Ensenada, BC, México.

Scientific Reports

|July 17, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Octopus maya embryos inherit reactive oxygen species (ROS) and antioxidants from their mothers. Embryos possess a robust antioxidant system to manage ROS, but their high metabolism limits energy for further defense against environmental stressors.

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Establishing an Octopus Ecosystem for Biomedical and Bioengineering Research

Establishing an Octopus Ecosystem for Biomedical and Bioengineering Research

Published on: September 22, 2021

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Analysis of Oxidative Stress in Zebrafish Embryos

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Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

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Establishing an Octopus Ecosystem for Biomedical and Bioengineering Research

Establishing an Octopus Ecosystem for Biomedical and Bioengineering Research

Published on: September 22, 2021

Area of Science:

Marine Biology
Developmental Biology
Biochemistry

Background:

Octopus maya embryos inherit reactive oxygen species (ROS) from maternal yolk synthesis.
Understanding embryonic antioxidant capacity is crucial for survival and development.

Purpose of the Study:

To quantify maternal ROS transfer to Octopus maya embryos.
To assess embryonic capacity for neutralizing ROS during development.
To investigate the relationship between metabolism, ROS production, and antioxidant gene expression in embryos.

Main Methods:

Analysis of carbonylated proteins and lipid peroxidation (LPO) in embryos.
Measurement of glutathione (GSH) levels.
Monitoring of respiratory metabolism and ROS production.

Quantification of antioxidant gene expression (CAT, SOD, HIf-1A).

Main Results:

Octopus maya females transfer ROS (via carbonylated proteins and LPO) and antioxidants (GSH) to embryos.
Embryos express antioxidant genes (CAT, SOD) early, enabling ROS neutralization.
Embryonic metabolic rate is high, leading to significant ROS production, with limited capacity for increased energy expenditure.
High HIf-1A expression indicates circulatory system development during organogenesis.

Conclusions:

Octopus maya embryos possess a well-developed antioxidant system to manage endogenous and maternal ROS.
High metabolic rates and inherent biological limitations restrict the embryos' ability to combat additional oxidative stress from environmental factors.