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Published on: July 8, 2020
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IgA class-switched CD27-CD21+ B cells in IgA nephropathy
Anna Popova1,2,3, Baiba Slisere4,5, Karlis Racenis1,2,6
1Department of Nephrology, Pauls Stradins Clinical University Hospital, Riga, Latvia.
Summary
This study reveals that in Immunoglobulin A nephropathy (IgAN), specific B cell populations, particularly IgA+CD27- cells, are linked to pathogenic antibody production. These findings suggest mucosal immune dysregulation drives B cell activation in IgAN.
Area of Science:
- Nephrology
- Immunology
- Cell Biology
Background:
- Immunoglobulin A nephropathy (IgAN) involves galactose-deficient IgA1 (GdIgA1) antibodies.
- B cells are central to IgAN, but their activation pathways and IgA secretion sources are unclear.
Purpose of the Study:
- To investigate B cell activation pathways in IgAN.
- To identify the source of pathogenic GdIgA1 antibodies.
Main Methods:
- Flow cytometry analysis of peripheral blood B cells in IgAN patients and controls.
- Focus on IgA-expressing B cells and their markers (CD27, CD21+).
Main Results:
- IgAN patients show increased IgA+CD27- B cells and IgA+ antibody-secreting cells, correlating with serum IgA.
- Both IgA+ plasmablasts and CD27- B cells co-express GdIgA1.
- A correlation between serum lipopolysaccharide and IgA+CD27- B cells suggests mucosal involvement.
Conclusions:
- Dysregulated mucosal immunity may drive de novo B cell activation, leading to IgA+CD27- B cells and IgA-producing plasmablasts in IgAN.
- These findings integrate B cells into IgAN pathogenesis, offering targets for biomarkers and therapies.
