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  11. Dihydroquercetin And Biochaga Reduce H2o2-induced Dna Damage In Peripheral Blood Mononuclear Cells Of Obese Women In Vitro-a Pilot Study

Dihydroquercetin and biochaga reduce H2O2-induced DNA damage in peripheral blood mononuclear cells of obese women in vitro-a pilot study

Lada Živković1, Andrea Pirković2, Dijana Topalović1

  • 1Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, 11000, Belgrade, Serbia.

Mutagenesis
|July 18, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Dihidroquercetin (DHQ) and biochaga (B) demonstrated potential in reducing hydrogen peroxide (H2O2)-induced DNA damage in obese individuals

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oxidative Stress Research

Background:

  • Obesity is linked to systemic oxidative stress, characterized by increased free radicals and reduced antioxidant defenses.
  • Hydrogen peroxide (H2O2) is a key mediator of DNA damage in oxidative stress conditions.

Purpose of the Study:

  • To evaluate the DNA protective effects of dihidroquercetin (DHQ) and biochaga (B) against H2O2-induced DNA damage in peripheral blood mononuclear cells (PBMCs).
  • To compare the efficacy of DHQ and B under pre-treatment and post-treatment conditions in obese subjects and controls.

Main Methods:

  • In vitro induction of DNA damage using H2O2 in PBMCs from obese individuals and controls.
  • Evaluation of DNA damage using the comet assay.
  • Assessment of antioxidant properties including reducing power and DPPH/∙OH scavenging activity of DHQ and B.
Keywords:
1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activitycomet assayhydroxyl radical scavenging activity

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Main Results:

  • Both DHQ and B significantly reduced H2O2-induced DNA damage in PBMCs from obese subjects, particularly with pre-treatment.
  • DHQ exhibited strong antioxidant properties, including reducing power and scavenging activity, contributing to DNA protection.
  • Combined DHQ and B showed enhanced reducing power and DPPH scavenging effects.

Conclusions:

  • DHQ and B show promise in mitigating H2O2-induced DNA damage in PBMCs from obese individuals.
  • These compounds may serve as valuable tools for future research into combating oxidative damage-related conditions.
the ferric reducing anti-oxidant power (FRAP) assay