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Cancer marker TNFRSF1A: From single‑cell heterogeneity of renal cell carcinoma to functional validation

  • 0Department of Ultrasound, Ningbo Yinzhou No. 2 Hospital, Ningbo, Zhejiang 315153, P.R. China.
Oncology Letters +

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July 18, 2024

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July 18, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Tumor microenvironment (TME) in clear cell renal cell carcinoma (ccRCC) involves TNF signaling. Targeting TNF receptor superfamily member 1A (TNFRSF1A) may inhibit ccRCC progression and enhance precision medicine.

Area Of Science

  • Oncology
  • Immunology
  • Molecular Biology

Background

  • Renal cell carcinoma (RCC) progression, metastasis, and treatment response are influenced by the tumor microenvironment (TME).
  • Understanding TME crosstalk is crucial for developing effective ccRCC therapies.

Purpose Of The Study

  • Investigate the TME's role in RCC, focusing on clear cell RCC (ccRCC).
  • Construct a ccRCC crosstalk network.
  • Evaluate TNF receptor superfamily member 1A (TNFRSF1A) as a potential therapeutic target for ccRCC.

Main Methods

  • Single-cell data analysis (GSE152938 dataset) to identify key cellular components in ccRCC TME.
  • Cell-cell communication analysis to map ccRCC microenvironment networks.
  • Database analysis (TCGA, CPTPAC) for TNF receptor genes, focusing on TNFRSF1A.
  • In vitro experiments (siRNA silencing of TNFRSF1A in 786-O cells) including proliferation, migration, invasion, cell cycle, and apoptosis assays.

Main Results

  • The TNF signaling pathway plays a critical role in ccRCC development.
  • Specific crosstalk between TNF and TNFRSF1A was identified within the TME.
  • TNFRSF1A silencing inhibited ccRCC cell proliferation, migration, and invasion.

Conclusions

  • TNFRSF1A promotes ccRCC progression, suggesting it as a viable therapeutic target.
  • Targeting TNFRSF1A could disrupt tumor progression and advance precision medicine for RCC patients.
  • Understanding TME crosstalk, particularly within the TNF pathway, is key for improving RCC prognosis.

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