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Related Experiment Videos

Rabies encephalitis: immunohistochemical investigations.

W Feiden, U Feiden, L Gerhard

    Clinical Neuropathology
    |July 1, 1985
    PubMed
    Summary

    This study used immunohistochemistry to detect rabies virus ribonucleoprotein (RNP) in human brain tissue. Rabies virus antigen was found in various brain regions and glial cells, revealing more infected cells than previously identified by Negri bodies.

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    Area of Science:

    • Neurology
    • Virology
    • Immunohistochemistry

    Background:

    • Rabies encephalitis is a fatal neurological disease caused by the rabies virus.
    • Diagnosis often relies on identifying Negri bodies, but their presence can be inconsistent.
    • Understanding the distribution of viral antigens is crucial for comprehending disease pathogenesis.

    Purpose of the Study:

    • To investigate the distribution of rabies virus ribonucleoprotein (RNP) antigen in human rabies encephalitis using immunohistochemistry.
    • To compare the sensitivity of immunohistochemistry with traditional methods for detecting rabies virus infection.

    Main Methods:

    • Studied three cases of human rabies encephalitis.
    • Employed immunohistochemistry with a specific antiserum to rabies RNP and the peroxidase-antiperoxidase method.

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  • Examined various cerebral regions and the spinal cord.

    • Main Results:

    • Rabies virus RNP was detected in all studied cerebral regions and the spinal cord.
    • Virus antigen showed predilection for the diencephalon and brain stem, correlating with clinical course.
    • Antigen was found in nerve cell bodies, processes, and glial cells, notably interfascicular oligodendroglia.
    • Immunohistochemistry identified significantly more infected cells than Negri bodies in hematoxylin-eosin stained sections.
    • Viral antigen was present in the cytoplasm, not exclusively within Negri bodies.

    Conclusions:

    • Immunohistochemistry is a more sensitive method for detecting rabies virus antigen in human encephalitis.
    • The distribution pattern suggests specific neuronal and glial targets, potentially indicating a route of infection.
    • Findings contribute to understanding the pathoclisis and pathogenesis of rabies virus infection.