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Updated: Jun 20, 2025
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
Published on: June 30, 2010
Protocol to study early lung developmental branching in mouse embryos using explant culture
Salli Talvi1, Johanna Jokinen1, Pia Rantakari2
1Department of Life Technologies, University of Turku, 20014 Turku, Finland; Medicity Research Laboratory, University of Turku, 20014 Turku, Finland; InFLAMES Research Flagship, University of Turku, 20014 Turku, Finland.
Abstract:
Mouse lung branching morphogenesis creates epithelial tree structures required for respiration. Here, we present a protocol for studying mouse lung developmental branching using lung explant cultures. We describe steps for isolating lungs with a video at embryonic day 12.5 (E12.5) and culturing as an explant for 2 days. We also detail procedures for microscopic imaging on days 0-2 and analysis of peripheral lung buds. This technique has the potential to investigate lung development in various conditions. For complete details on the use and execution of this protocol, please refer to Talvi et al.1.
