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Related Concept Videos

Drug Distribution: Plasma Protein Binding01:29

Drug Distribution: Plasma Protein Binding

5.5K
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
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Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

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Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
161
Physiological Pharmacokinetic Models: Assumption with Protein Binding01:13

Physiological Pharmacokinetic Models: Assumption with Protein Binding

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Physiological models with protein binding in pharmacokinetics offer a sophisticated approach to understanding drug disposition. These models consider drug-protein interactions, enabling them to effectively predict drug concentrations in different organs and tissues. This precision aids in accurate drug dosing, providing a significant advantage over conventional models. A key process within these models is equilibration, which ensures that drug concentrations achieve a steady state within the...
39
Protein-Drug Binding: Mechanism and Kinetics01:16

Protein-Drug Binding: Mechanism and Kinetics

392
Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
392
Hepatic Drug Clearance: Effect of Protein Binding01:09

Hepatic Drug Clearance: Effect of Protein Binding

184
Hepatic clearance is influenced by protein binding based on the drug's extraction ratio. Drugs with high extraction ratios are considered flow-limited and remain unaffected by protein binding during hepatic clearance. On the other hand, drugs with low extraction ratios may be impacted by plasma protein binding, although the extent of this influence depends on the fraction of the drug bound.
For low-extraction-ratio drugs that are less than 80% protein-bound, minor changes in protein binding...
184
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
12.9K

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A method for estimation of plasma protein binding using diffusion ordered NMR spectroscopy (DOSY).

Rachel Taylor1, Thomas Swift2, David Wilkinson3

  • 1Institute of Cancer Therapeutics, University of Bradford Richmond Road BD7 1DP UK.

RSC Medicinal Chemistry
|July 19, 2024
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Summary
This summary is machine-generated.

This study introduces Diffusion Ordered Spectroscopy (DOSY) as a rapid method to assess drug plasma protein binding (PPB). This technique aids in early drug discovery by evaluating drug-protein interactions, specifically with bovine serum albumin (BSA).

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Area of Science:

  • Medicinal Chemistry
  • Pharmacology
  • Analytical Chemistry

Background:

  • Plasma protein binding (PPB) significantly influences drug pharmacokinetics and pharmacodynamics.
  • Early assessment of PPB is crucial during lead optimization for predicting drug behavior.
  • Existing methods for PPB determination can be time-consuming, necessitating faster approaches.

Purpose of the Study:

  • To present Diffusion Ordered Spectroscopy (DOSY) as a rapid and straightforward technique for evaluating drug PPB.
  • To demonstrate the utility of DOSY for assessing drug interactions with plasma proteins.
  • To utilize bovine serum albumin (BSA) as a model protein for PPB evaluation using DOSY.

Main Methods:

  • Utilized Diffusion Ordered Spectroscopy (DOSY), an NMR technique measuring molecular diffusion.
  • Applied DOSY to quantify the diffusion of drug molecules in the presence of bovine serum albumin (BSA).
  • Correlated changes in diffusion coefficients with drug binding to BSA to determine PPB.

Main Results:

  • DOSY successfully differentiated between free and bound drug molecules based on diffusion rates.
  • The method provided a rapid and accurate assessment of drug-bovine serum albumin (BSA) binding.
  • Demonstrated the potential of DOSY for early-stage PPB screening in drug discovery.

Conclusions:

  • Diffusion Ordered Spectroscopy (DOSY) offers a valuable tool for rapid and efficient evaluation of plasma protein binding (PPB).
  • This NMR-based method aids in the early stages of drug discovery by providing critical pharmacokinetic and pharmacodynamic insights.
  • The use of bovine serum albumin (BSA) as a model system validates DOSY's applicability for assessing drug-protein interactions.