Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Domains01:18

Membrane Domains

5.4K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
5.4K
Membrane Fluidity01:26

Membrane Fluidity

11.1K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.1K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

6.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
6.9K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.1K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.1K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.2K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.0K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lenacapavir-induced lattice hyperstabilization is central to HIV-1 capsid failure at the nuclear pore complex and in the cytoplasm.

eLife·2026
Same author

Systematic bottom-up coarse-graining of hydrated excess proton transport across scales.

Nature computational science·2026
Same author

Mechanism of HIV-1 Capsid Rupture and Uncoating by Reverse Transcription.

bioRxiv : the preprint server for biology·2026
Same author

Physical Confinement Modulates the Rate-Limiting Transition in the Release of Phosphate from Actin Filaments.

bioRxiv : the preprint server for biology·2026
Same author

Hydration-Controlled Proton Transport in Respiratory Complex I.

Journal of the American Chemical Society·2026
Same author

Mechanistic insights into lenacapavir-induced off-pathway HIV-1 capsid assembly.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Whole-Embryo 3D Quantification Reveals Conserved Topological Design and Scaling of Germ Layers in Xenopus.

bioRxiv : the preprint server for biology·2026
Same journal

scRNA-seq and genomics analyses reveal key mechanisms of inverted papilloma-associated sinonasal squamous cell carcinoma malignant transformation.

bioRxiv : the preprint server for biology·2026
Same journal

M1C IS NECESSARY FOR DARAXONRASIB RESISTANCE OF NSCLC KRAS(G12C) MUTANT CELLS.

bioRxiv : the preprint server for biology·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2025

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering
10:56

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

Published on: April 12, 2024

1.0K

Lipid Organization by the Caveolin-1 Complex.

Korbinian Liebl1, Gregory A Voth1

  • 1Department of Chemistry, Chicago Center for Theoretical Chemistry, Institute for Biophysical Dynamics, and James Franck Institute, The University of Chicago, Chicago, IL 60637.

Biorxiv : the Preprint Server for Biology
|July 19, 2024
PubMed
Summary
This summary is machine-generated.

Caveolin-1 (CAV1) oligomers remodel cell membranes and manage cholesterol. Molecular dynamics simulations reveal how CAV1-8S complexes bend membranes and extract cholesterol, with palmitoylation enhancing this process.

Keywords:
Caveolin-1Martini CG simulationMetadynamics simulationscholesterol accumulationpalmitoylation

More Related Videos

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.1K
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
10:19

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: August 25, 2022

3.4K

Related Experiment Videos

Last Updated: Jun 20, 2025

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering
10:56

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

Published on: April 12, 2024

1.0K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.1K
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
10:19

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: August 25, 2022

3.4K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Caveolins, such as CAV1, are lipid-binding proteins involved in membrane remodeling.
  • Caveolins oligomerize into 8S-complexes, which further assemble into caveolae, structures dependent on cholesterol concentration.
  • The precise molecular mechanisms of membrane remodeling and cholesterol handling by caveolins remain unclear.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which the CAV1 8S-complex induces membrane bending and cholesterol accumulation.
  • To investigate the role of CAV1 palmitoylation in these processes.
  • To validate simulation forcefield accuracy for membrane bending.

Main Methods:

  • Atomistic Molecular Dynamics (MD) simulations.
  • Advanced sampling techniques.
  • Backmapping from coarse-grained to all-atom models.

Main Results:

  • The CAV1-8S complex bends lipid bilayers and accumulates cholesterol within its structure.
  • Palmitoylation of CAV1 enhances membrane bending and cholesterol accumulation.
  • Atomistic simulations show localized membrane bending, contrasting with overestimation by the Martini v2 coarse-grained forcefield.

Conclusions:

  • The CAV1-8S complex actively remodels membranes and can extract cholesterol from the lipid bilayer.
  • Palmitoylation is a key factor modulating CAV1's interaction with membranes and cholesterol.
  • Atomistic MD simulations provide a more accurate representation of membrane bending by CAV1 compared to certain coarse-grained models.