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Updated: Jun 20, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
De-novo assembly of four rail (Aves: Rallidae) genomes: A resource for comparative genomics
Julien Gaspar1,2, Steve A Trewick1, Gillian C Gibb1
1School of Food Technology and Natural Sciences, Wildlife and Ecology Group Massey University Palmerston North New Zealand.
Abstract:
Rails are a phenotypically diverse family of birds that includes 130 species and displays a wide distribution around the world. Here we present annotated genome assemblies for four rails from Aotearoa New Zealand: two native volant species, pūkeko Porphyrio melanotus and mioweka Gallirallus philippensis, and two endemic flightless species takahē Porphyrio hochstetteri and weka Gallirallus australis. Using the sequence read data, heterozygosity was found to be lowest in the endemic flightless species and this probably reflects their relatively small populations. The quality checks and comparison with other rallid genomes showed that the new assemblies were of good quality. This study significantly increases the number of available rallid genomes and will enable future genomic studies on the evolution of this family.
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