Novel hollow-electrode glow discharge mass spectrometry for the quantitative analysis of protein content in food

Protein content in food is an important indicator of nutritional value and food safety. Therefore, it is of great significance to accurately detect protein content in food. In this work, a combustion furnace and novel hollow-electrode glow discharge ion source-quadrupole mass spectrometry (HGD-MS) were designed, which were used to construct a "combustion furnace + mass spectrometry" experimental platform to detect the protein content in food. Five food standard samples were selected for the analysis. The food samples were combusted in the combustion furnace at a high temperature (1300 °C) in an oxygen-rich environment. The gas products were passed into the novel hollow electrode glow discharge ion source-quadrupole mass spectrometer. A standard curve of y = 635.06x + 11 082, R² = 0.9994 was plotted by detecting the NO⁺ ion intensity at a relative standard deviation (RSD) of 1.8% to 5.7%. Using the same method, food samples no. 6 and 7 were combusted and NO⁺ ion intensity was measured to verify the accuracy of the quantitation curve. Subsequently, the protein content was determined using a nitrogen-to-protein conversion factor of 6.25. This method provides a rapid, accurate, and environmentally friendly approach for determining protein content in food.