Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of Key Candidate Protein Biomarkers in Early-Stage Nonsmall Cell Lung Carcinoma through Quantitative Proteomics.

Journal of proteome research·2025
Same author

Current Trends in Biomedical Hydrogels: From Traditional Crosslinking to Plasma-Assisted Synthesis.

Polymers·2022
Same author

Interplay between Alba and Cren7 Regulates Chromatin Compaction in <i>Sulfolobus solfataricus</i>.

Biomolecules·2022
Same author

Different Proteins Mediate Step-Wise Chromosome Architectures in <i>Thermoplasma acidophilum</i> and <i>Pyrobaculum calidifontis</i>.

Frontiers in microbiology·2020
Same author

Dynamic chromatin organization in the cell.

Essays in biochemistry·2019

Related Experiment Video

Updated: Jun 20, 2025

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

22.5K

Atomic Force Microscopy Characterization of Reconstituted Protein-DNA Complexes.

Marc Kenneth M Cajili1, Eloise I Prieto2

  • 1Leiden Institute of Chemistry, Leiden University, Leiden, Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|July 19, 2024
PubMed
Summary

Atomic force microscopy (AFM) visualizes biomolecular structures. This study details an AFM protocol for analyzing DNA-protein interactions and chromatin assembly in vitro, successfully applied to archaeal proteins.

Keywords:
ArchaeaAtomic force microscopyChromatinChromatin reconstitutionNucleoidNucleoid-associated protein (NAP)

More Related Videos

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

8.7K
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.1K

Related Experiment Videos

Last Updated: Jun 20, 2025

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

22.5K
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

8.7K
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.1K

Area of Science:

  • Biophysics
  • Molecular Biology
  • Structural Biology

Background:

  • Atomic force microscopy (AFM) offers high-resolution imaging of biomolecular complexes.
  • Characterizing protein-DNA interactions is crucial for understanding chromatin structure and function.
  • In vitro reconstitution allows controlled study of chromatin assembly.

Purpose of the Study:

  • To present a detailed protocol for AFM analysis of reconstituted chromatin.
  • To demonstrate the application of this method for studying chromatin architectural proteins.
  • To analyze the binding behavior and structural units assembled on DNA.

Main Methods:

  • In vitro reconstitution of chromatin using purified DNA and proteins.
  • Preparation of samples for Atomic Force Microscopy (AFM).
  • AFM imaging and subsequent structural analysis of assembled chromatin.

Main Results:

  • Successful application of the AFM protocol to chromatin architectural proteins from Sulfolobus solfataricus.
  • Detailed structural characterization of protein-DNA complexes and assembled chromatin units.
  • Insights into the binding behavior of specific chromatin architectural proteins.

Conclusions:

  • The described AFM method provides a robust approach for structural analysis of reconstituted chromatin.
  • This technique is valuable for characterizing DNA-protein interactions and chromatin assembly.
  • The study successfully applied AFM to archaeal chromatin architectural proteins, highlighting its broad applicability.