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Related Concept Videos

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  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Identification And Validation Of Basement Membrane-related Genes Predicting Prognosis And Immune Infiltration Associated With Bladder Cancer

Identification and validation of basement membrane-related genes predicting prognosis and immune infiltration associated with bladder cancer

Fie Lai1, Lin He2, Thongher Lia1

  • 1Department of Urology Surgery, Chengdu Second People's Hospital, Chengdu, Sichuan, China.

Medicine
|July 19, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

This study identifies three basement membrane genes to predict bladder cancer (BC) progression. A high-risk group showed poorer survival, indicating these genes are effective BC prognostic tools.

Area of Science:

  • Oncology
  • Genomics
  • Biomarkers

Background:

  • Bladder cancer (BC) poses a significant threat, especially during muscle invasion, with limited treatment advancements.
  • Basement membrane (BM) integrity is crucial in cancer progression, yet its role in BC prognosis requires further elucidation.

Purpose of the Study:

  • To develop and validate a prognostic gene signature based on basement membrane (BM)-associated genes for bladder cancer (BC).
  • To investigate the correlation between the identified gene signature and tumor immune infiltration in BC.

Main Methods:

  • Utilized COX regression and LASSO analysis on TCGA database to identify BM-related genes.
  • Validated the prognostic value of BM genes using GSE32548, GSE129845 datasets, and immunohistochemistry.
  • Performed Spearman correlation and drug sensitivity analyses to assess risk score associations.

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Main Results:

  • A three-gene signature (EPEMP1, GPC2, ITGA3) was constructed, predicting BC prognosis.
  • High-risk patients exhibited significantly worse overall survival (OS) across multiple cohorts.
  • Risk score positively correlated with specific immune cell types (B cell naïve, M2 macrophages) and immune scores.

Conclusions:

  • The identified three-gene signature serves as a novel and effective tool for risk stratification in bladder cancer (BC) patients.
  • This BM-gene based signature aids in predicting BC progression and understanding tumor immune microenvironment interactions.