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An immunochemical method for fingerprinting Clostridium difficile.

J Sharp, I R Poxton

    Journal of Immunological Methods
    |November 7, 1985
    PubMed
    Summary
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    Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) offers a stable method for fingerprinting Clostridium difficile strains. This technique aids in understanding the epidemiology of C. difficile-associated diarrheal diseases.

    Area of Science:

    • Microbiology
    • Immunology
    • Molecular Biology

    Background:

    • Clostridium difficile is a significant cause of infectious diarrhea.
    • Accurate strain identification is crucial for epidemiological studies.
    • Existing methods for C. difficile typing may have limitations.

    Purpose of the Study:

    • To evaluate the utility of SDS-PAGE coupled with immunoblotting for Clostridium difficile strain fingerprinting.
    • To assess the stability of Clostridium difficile antigens under various conditions.

    Main Methods:

    • Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was employed.
    • Proteins were transferred to nitrocellulose membranes.
    • Antisera were used for probing (immunoblotting).

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  • Antigen stability was tested during subculture and antigen preparation.

    • Main Results:

    • SDS-PAGE and immunoblotting proved effective for fingerprinting Clostridium difficile.
    • Clostridium difficile antigens demonstrated remarkable stability in vitro and in vivo.
    • Minor variations in antigen extraction did not significantly alter immunoblot patterns.
    • Individuals can harbor multiple C. difficile strains concurrently.

    Conclusions:

    • SDS-PAGE with immunoblotting is a potentially valuable tool for Clostridium difficile strain identification.
    • The stability of C. difficile antigens supports the reliability of this fingerprinting method.
    • This technique can contribute to studying the epidemiology of C. difficile-associated diarrhea.
    • Recommendations for future studies include subculturing multiple colonies from primary isolation plates.