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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Jun 20, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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A programmable sensitive platform for pathogen detection based on CRISPR/Cas12a -hybridization chain reaction-poly

Haolin Sun1, Xiaoyu Zhang1, Hainan Ma1

  • 1School of Public Health, Jilin University, Changchun, Jilin, 130021, PR China.

Analytica Chimica Acta
|July 19, 2024
PubMed
Summary

A new CRISPR-based platform enhances pathogenic bacteria detection sensitivity using hybridization chain reaction and a low-cost nanoprobe. This innovation improves accuracy for disease control and safety applications.

Keywords:
BiosensorCRISPR/Cas12aFluorescentHybridization chain reactionPathogenPoly T-Copper probe

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanotechnology

Background:

  • Rapid and sensitive detection of pathogenic bacteria is vital for public health.
  • CRISPR/Cas12a systems show potential for bacterial diagnosis but face sensitivity limitations.

Purpose of the Study:

  • To develop a versatile and highly sensitive pathogen sensing platform (HTCas12a).
  • To improve the sensitivity and reduce the cost of CRISPR-based bacterial detection.

Main Methods:

  • Utilized the CRISPR/Cas12a system combined with hybridization chain reaction (HCR).
  • Employed a Poly T-copper fluorescence nanoprobe for signal amplification.
  • Validated specificity against various pathogen nucleic acid fragments.

Main Results:

  • Achieved enhanced sensitivity through HCR and the Poly-T-Cu reporter probe.
  • Demonstrated specific recognition of target nucleic acid fragments.
  • Established a linear correlation between fluorescence and target quantity with low detection limits (23.36 fM for DNA, 4.17 CFU/mL for S. aureus).
  • Reduced experiment cost to under one dollar per sample.

Conclusions:

  • The HTCas12a system provides a sensitive, specific, and cost-effective platform for pathogen detection.
  • This technology has broad applications in environmental monitoring, clinical diagnostics, and food safety.