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Two isoaccepting seryl tRNAs coded by separate mitochondrial genes in yeast.

E Colletti, L Frontali, C Palleschi

Molecular & General Genetics : MGG

|August 1, 1979

Summary

Researchers identified two genes for serine transfer RNAs (tRNAs) in yeast mitochondria using deletion mapping. One tRNAser gene is near the oli I region, and another is near the tRNAarg gene.

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Area of Science:

Mitochondrial genetics
Molecular biology
Yeast genetics

Background:

Saccharomyces cerevisiae (yeast) mitochondria contain four isoacceptor serine transfer RNAs (tRNAs).
At least two of these tRNA species originate from distinct genes.
Understanding tRNA gene organization is crucial for mitochondrial gene expression.

Purpose of the Study:

To locate and characterize the genes encoding serine transfer RNAs (tRNAs) in yeast mitochondria.
To differentiate between isoacceptor tRNAs and their corresponding genes.

Main Methods:

Reversed-phase chromatography to separate tRNA isoacceptors.
Deletion mapping to identify gene locations within the mitochondrial genome.
Fine mapping to precisely localize genes.

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Main Results:

Four isoacceptor mitochondrial tRNAs for serine were separated in S. cerevisiae.
Deletion mapping identified two distinct genes for tRNAser.
One gene, previously localized to the oli I region, codes for tRNAser2.
A second gene for tRNAser1 was found near other tRNA genes, specifically adjacent to the tRNAarg gene.

Conclusions:

Two distinct genes encode the identified serine tRNAs in yeast mitochondria.
The gene for tRNAser2 is located in the oli I region.
The gene for tRNAser1 is located near the tRNAarg gene, contributing to the understanding of mitochondrial tRNA gene cluster organization.