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Chitinase-functionalized UiO-66 framework nanoparticles active against multidrug-resistant Candida Auris.

Shaymaa A Ismail1, Bahgat Fayed2, Reda M Abdelhameed3

  • 1Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Institute, National Research Centre, P.O. 12622, 33 El Bohouth Street, Dokki, Giza, Egypt. shaymaaabdallaismail@gmail.com.

BMC Microbiology
|July 19, 2024
PubMed
Summary

This study enhances chitinase production and immobilizes it on UiO-66, significantly boosting its antifungal activity against the resistant yeast Candida auris (C. auris). This novel approach offers a promising new strategy for combating C. auris infections.

Keywords:
Candida aurisTalaromyces variansChitinaseSSW3UiO66

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Area of Science:

  • Biotechnology and Biochemistry
  • Materials Science
  • Antimicrobial Research

Background:

  • Candida auris (C. auris) is an emerging multidrug-resistant yeast pathogen causing significant outbreaks.
  • The cell wall component chitin is crucial for C. auris antifungal resistance.
  • Enzyme immobilization on nanostructures can enhance enzyme activity and stability.

Purpose of the Study:

  • To investigate the potential of chitinase immobilized on UiO-66 as an antifungal agent against C. auris.
  • To optimize chitinase production and characterize the UiO-66 framework.
  • To evaluate the impact of immobilization on chitinase activity, stability, and antifungal efficacy.

Main Methods:

  • Optimized chitinase production from Talaromyces varians SSW3 using Plackett-Burman and Box-Behnken designs.
  • Synthesized and characterized UiO-66 nanomaterial using SEM, TEM, XRD, FTIR, particle size analysis, and zeta sizing.
  • Immobilized chitinase onto UiO-66 and assessed enzyme kinetics, stability, and antifungal activity against C. auris.

Main Results:

  • Chitinase yield was significantly enhanced to 120.41 U/g ds.
  • UiO-66 nanoparticles (70.42 nm) were successfully synthesized and characterized.
  • Immobilization on UiO-66 improved chitinase activity (2-fold Vmax increase, 38-fold Km decrease) and stability, and markedly boosted antifungal efficacy against C. auris (MIC50 reduced from 5.582 to 0.89 U/mL).

Conclusions:

  • Immobilizing chitinase on UiO-66 is an effective strategy to enhance its antifungal properties.
  • This approach presents a novel and potent alternative for combating the challenging pathogen C. auris.
  • The study highlights the potential of enzyme-nanomaterial conjugates in antimicrobial applications.