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Bioassays for Monitoring Insecticide Resistance

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Published on: December 30, 2010

Transcriptomic Response to Pyrethroid Treatment in Closely Related Bed Bug Strains Varying in Resistance.

Chloé Haberkorn^1,2, Zaïnab Belgaïdi¹, Romain Lasseur²

¹CNRS, VetAgro Sup, UMR 5558, Laboratoire de Biométrie et Biologie Évolutive, Universite Lyon 1, Villeurbanne, France.

Genome Biology and Evolution

|July 20, 2024

Summary

This study identified only 24 constitutive differentially expressed genes between pyrethroid-susceptible and resistant bed bug strains. Resistant strains showed increased expression of cuticular protein genes, suggesting qualitative cuticle changes, not thickness alterations.

Keywords:

Cimex lectularius RNA-seq insecticide resistance transcriptome

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Published on: January 15, 2020

Area of Science:

Entomology
Molecular Biology
Genomics

Background:

The common bed bug (Cimex lectularius) is a significant human ectoparasite.
Global resurgence is linked to globalization and widespread insecticide resistance.
Previous transcriptome studies often used geographically distant strains, complicating candidate gene identification.

Purpose of the Study:

To compare transcriptomes of closely related susceptible and resistant bed bug strains.
To identify constitutive differentially expressed genes related to pyrethroid resistance.
To investigate gene expression changes before and after insecticide exposure.

Main Methods:

Transcriptome sequencing of two closely related Cimex lectularius strains with differing pyrethroid susceptibility.
Analysis of gene expression profiles before and after insecticide exposure.
Comparison of identified candidate loci with genetically differentiated loci.

Main Results:

Only 24 genes showed constitutive differential expression between strains.
Resistant strains exhibited higher expression of cuticular protein genes, without significant changes in cuticle thickness.
Insecticide exposure induced numerous genes, primarily involved in detoxification pathways.
Limited overlap was found between transcriptome candidates and previously identified highly differentiated genetic loci.

Conclusions:

Constitutive genetic differences related to pyrethroid resistance in bed bugs are minimal.
Qualitative changes in the cuticle, rather than thickness, may play a role in resistance.
Insecticide exposure strongly induces detoxification gene expression.
Discrepancies with previous studies highlight the importance of strain selection in genetic research.