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A new method for quantification of tomatine-enriched extracts.

Catarina Faria-Silva1,2, Carla V Eleutério1, Pedro Simões2

  • 1iMed.ULisboa, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal.

Journal of the Science of Food and Agriculture
|July 20, 2024
PubMed
Summary
This summary is machine-generated.

This study optimized a selective high-performance liquid chromatography (HPLC) method for quantifying tomatine, a beneficial compound in green tomato extracts. The validated method is simple, rapid, and suitable for routine analysis of tomatine in agro-food waste.

Keywords:
HPLC method validationHPLC quantificationUV detectionalpha‐tomatine; green tomatoessteroidal glycoalkaloids

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Area of Science:

  • Agricultural Chemistry
  • Analytical Chemistry
  • Food Science

Background:

  • Green tomato extracts contain tomatine, a bioactive glycoalkaloid with potential health benefits.
  • Agro-food waste valorization is crucial for sustainable practices.
  • High-performance liquid chromatography (HPLC) is a standard technique for bioactive compound quantification.

Purpose of the Study:

  • To optimize and validate a selective HPLC method for tomatine analysis.
  • To quantify tomatine extracted from green tomatoes using subcritical water extraction.
  • To establish a simple and rapid analytical protocol for routine use.

Main Methods:

  • Optimization and validation of an HPLC method with a diode array detector (DAD).
  • Utilized an InertSustain Phenyl analytical column (250 mm × 4.6 mm, 5 μm).
  • Employed a mobile phase of acetonitrile and potassium dihydrogen phosphate buffer (20 mmol L-1, pH 3) at 205 nm.

Main Results:

  • The method demonstrated excellent linearity (R2 = 0.9999) over a concentration range of 50-580 μg mL-1.
  • High precision was observed with relative standard deviations (RSD%) of 1.4% for standards and 0.9% for extracts.
  • The method achieved a mean recovery of 105%, with limits of detection (LOD) and quantitation (LOQ) at 8.0 and 24.1 μg mL-1, respectively.

Conclusions:

  • The developed HPLC method is effective for quantifying tomatine in green tomato extracts.
  • The method is simple, rapid, and suitable for routine analytical protocols.
  • This contributes to the valorization of agro-food waste by enabling efficient analysis of bioactive compounds.