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Updated: Jun 20, 2025

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
The abundant fraction of soil microbiomes regulates the rhizosphere function in crop wild progenitors
Miguel de Celis1, María José Fernández-Alonso2,3, Ignacio Belda4
1Departamento de Suelo, Planta y Calidad Ambiental, Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Abstract:
The rhizosphere influence on the soil microbiome and function of crop wild progenitors (CWPs) remains virtually unknown, despite its relevance to develop microbiome-oriented tools in sustainable agriculture. Here, we quantified the rhizosphere influence-a comparison between rhizosphere and bulk soil samples-on bacterial, fungal, protists and invertebrate communities and on soil multifunctionality across nine CWPs at their sites of origin. Overall, rhizosphere influence was higher for abundant taxa across the four microbial groups and had a positive influence on rhizosphere soil organic C and nutrient contents compared to bulk soils. The rhizosphere influence on abundant soil microbiomes was more important for soil multifunctionality than rare taxa and environmental conditions. Our results are a starting point towards the use of CWPs for rhizosphere engineering in modern crops.
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