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MCM8 promotes lung cancer progression through upregulating DNAJC10
Lei Cao1, Hongsheng Liu1, Zhijun Han1
1Department of Thoracic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
Journal of Cellular and Molecular Medicine
|July 20, 2024
Summary
High MCM8 (minichromosome maintenance 8) expression drives lung cancer (LC) progression and metastasis by regulating DNAJC10. This MCM8/DNAJC10 axis presents a potential therapeutic target and diagnostic biomarker for LC patients.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- MCM8 (minichromosome maintenance 8) is a helicase involved in DNA replication and tumorigenesis.
- MCM8 is upregulated in various human cancers, including lung cancer (LC), but its role in LC progression remains unclear.
Purpose of the Study:
- To investigate the function and mechanism of MCM8 in lung cancer development.
- To identify potential therapeutic targets and diagnostic biomarkers for lung cancer.
Main Methods:
- Analysis of MCM8 expression in LC cells and tissues.
- In vitro and in vivo experiments involving MCM8 knockdown and overexpression.
- Gene array and co-immunoprecipitation (CO-IP) assays to identify MCM8 targets.
- Analysis of The Cancer Genome Atlas (TCGA) database for MCM8 and DNAJC10 correlation.
Main Results:
- MCM8 was highly expressed in LC and associated with advanced tumor grade, metastasis, and poor prognosis.
- MCM8 knockdown inhibited LC cell growth and migration; MCM8 overexpression promoted cell cycle progression, migration, proliferation, and apoptosis.
- DNAJC10 was identified as a downstream target of MCM8, mediating its oncogenic effects.
- MCM8 and DNAJC10 expression were positively correlated in LC, with DNAJC10 upregulation also linked to poor survival.
Conclusions:
- The MCM8/DNAJC10 axis plays a crucial role in lung cancer development.
- MCM8 and DNAJC10 represent potential therapeutic targets and diagnostic biomarkers for lung cancer.
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