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Updated: Jun 20, 2025
Sample Preparation for Mass Cytometry Analysis
Published on: April 29, 2017
Implementation of flow cytometry testing on rare matrix samples: Special considerations and best practices when the
Katherine A Devitt1,2, Wolfgang Kern3, Malgorzata A Kajstura4
1Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington, Vermont, USA.
Abstract:
The publication of Clinical and Laboratory Standards Institute's guideline H62 has provided the flow cytometry community with much-needed guidance on development and validation of flow cytometric assays (CLSI, 2021). It has also paved the way for additional exploration of certain topics requiring additional guidance. Flow cytometric analysis of rare matrices, or unique and/or less frequently encountered specimen types, is one such topic and is the focus of this manuscript. This document is the result of a collaboration subject matter experts from a diverse range of backgrounds and seeks to provide best practice consensus guidance regarding these types of specimens. Herein, we define rare matrix samples in the setting of flow cytometric analysis, address validation implications and challenges with these samples, and describe important considerations of using these samples in both clinical and research settings.
