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Updated: Jun 20, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
Boosting resistant starch in rice: Bacterial inulin as a metabolic and glucose uptake modulator
Ravishankar Palanisamy1, Satheesh Kumar Subramanian2, Samuel Kuwaku Asiedu3
1Department of Neurosurgery, McGill University, Montreal, Quebec H3A 0G4, Canada; Department of Biotechnology, Periyar University, Salem, Tamil Nadu 636011, India; Rayakis, Energy and Environmental Consultancy, Periyar Street, Salem, Tamil Nadu 636 001, India.
Abstract:
Bacillus stercoris PSSR12 (B. stercoris PE), an isolate from rice field soils, was identified via 16s rRNA sequencing. The synthesis of the inulin and inulin producing enzyme (IPE) in B. stercoris PE was verified using SDS-PAGE and FTIR. This study aimed to assess the impact of B. stercoris PE treatment on in vitro inhibition of α-amylase and α-glucosidase from traditional and commercial rice varieties of South India. Additionally, the study investigated enzymatic inhibition and mRNA expression of starch synthesis genes (RAmy1a, GBSSIa, SBEIIa, and SBEIIb). Glucose transporter gene expression (GLUT1 and GLUT4) patterns were analyzed in 3T3-L1 adipocytes to evaluate glucose uptake in B. stercoris PE treated rice varieties. The application of B. stercoris PE enhanced grain quality by imparting starch ultra-structural rigidity, inhibiting starch metabolizing enzymes, and inducing molecular changes in starch synthesis genes. This approach holds promise for managing type II diabetes mellitus and potentially reducing insulin dependence.
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